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Stable Cell Line Generation

Introduction

High quality stable cell lines play an important role in biological research, including gene function studies, recombinant antibodies, drug discovery, and so on. Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected DNA into the genome. Currently, Lentivirus is a most wildly used method to generate a stable cell lines due to its capability for efficient integration of the transgene and wide infectivity. WZ Biosciences offers various services, including OE vector construction, OE lentivirus packaging, gene overexpression validation, OE stable cell lines generation, and so on. *OE: overexpression



Service Details

WZ Biosciences scientists have years of experience at stable cell lines generation, and can generate high quality stable cell lines for you. You can provide cell lines to us or you can select cell lines from WZ Biosciences’ cell bank.

Cat No.

Type

OE Cell Line Type

Price

Turnaround Time

WZ130001-1

Single Gene OE Cell Line Generation

Polyclonal

$2000/cell

4-6weeks

WZ130001-2

Monoclonal

$4000/cell

12weeks

WZ130002-1

Multiple Gene OE Cell Line Generation

Polyclonal

Quote

Inquire

WZ130002-2

Monoclonal

Quote

Inquire


*PS: If you provide OE cell lines to us, additional cost will be required.

*Interested in above OE Cell Line Generation services? Please feel free to contact us to “Request a quote”.


Related services: Cloning Services, Lentivirus packaging, Gene Overexpression Validation




The Principle of Stable Cells Generation

The foreign DNA/shRNA is cloned into an expression vector with containing one resistance gene. The recombinant vector is then introduced into the host cell and integrated into its chromosome, so that the foreign DNA/shRNA can be transmitted to daughter cells upon cell division. The stable cell line are selected with the resistant gene on plasmid. The most commonly used selection antibiotics for stable cell transfection are neomycin, blasticidin and puromycin.



维真生物稳定细胞系建立的原理






Advantages

1. Cells for stable cell lines generation is high quality.

2. All WZ Biosciences’ cell lines are purchased from the ATCC Cell Line Bank.

3. Manufacturing technology is stable. Strictly selection process and target gene expression verification.

4. High cell viability. Cell viability are measured in successive 5 generation after a stable cell line establishment.

5. Guarantee Production Quality. Functional validation is determined by RT- PCR.



Service Process



维真生物稳定细胞系建立服务流程




Example








Related Resorces


维真生物-慢病毒/LV技术手册

《Lentivirus Manual》