Stable Cell Line Generation
Introduction
High quality stable cell lines play an important role in biological research, including gene function studies, recombinant antibodies, drug discovery, and so on. Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected DNA into the genome. Currently, Lentivirus is a most wildly used method to generate a stable cell lines due to its capability for efficient integration of the transgene and wide infectivity. WZ Biosciences offers various services, including OE vector construction, OE lentivirus packaging, gene overexpression validation, OE stable cell lines generation, and so on. *OE: overexpression
Service Details
WZ Biosciences scientists have years of experience at stable cell lines generation, and can generate high quality stable cell lines for you. You can provide cell lines to us or you can select cell lines from WZ Biosciences’ cell bank.
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Cat No.
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Type
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OE Cell Line Type
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Price
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Turnaround Time
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WZ130001-1
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Single Gene OE Cell Line Generation
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Polyclonal
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$2000/cell
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4-6weeks
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WZ130001-2
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Monoclonal
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$4000/cell
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12weeks
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WZ130002-1
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Multiple Gene OE Cell Line Generation
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Polyclonal
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Quote
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Inquire
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WZ130002-2
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Monoclonal
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Quote
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Inquire
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*PS: If you provide OE cell lines to us, additional cost will be required.
*Interested in above OE Cell Line Generation services? Please feel free to contact us to “Request a quote”.
Related services： Cloning Services, Lentivirus packaging, Gene Overexpression Validation
The Principle of Stable Cells Generation
The foreign DNA/shRNA is cloned into an expression vector with containing one resistance gene. The recombinant vector is then introduced into the host cell and integrated into its chromosome, so that the foreign DNA/shRNA can be transmitted to daughter cells upon cell division. The stable cell line are selected with the resistant gene on plasmid. The most commonly used selection antibiotics for stable cell transfection are neomycin, blasticidin and puromycin.
Advantages
1. Cells for stable cell lines generation is high quality.
2. All WZ Biosciences’ cell lines are purchased from the ATCC Cell Line Bank.
3. Manufacturing technology is stable.
Strictly selection process and target gene expression verification.
4. High cell viability.
Cell viability are measured in successive 5 generation after a stable cell line establishment.
5. Guarantee Production Quality.
Functional validation is determined by RT- PCR.
Service Process
Example
Related Resorces
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《Lentivirus Manual》
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