WZ Biosciences are specialized in design and preparing viral based vectors in gene delivery for research and gene therapy purpose. We have many products and services. Our plasmid products include more than 17,000 ready for mammalian expression human ORF clones, more than 1000 human microRNA clones, gRNA expression clones targeting more than 14,000 mouse genes. We have hundreds of vectors for expression of ORF, microRNA, shRNA and gRNAs. These vectors can be used in preparing lentivirus, adenovirus and AAV virus. Our premade adenovirus products include more than 12,000 human ORF and more than 1000 human microRNA. Our ready to use AAV virus includes different fluorescent protein, channelrhodopsins, DREADDs, Cre, Flippase, and saCas9 in more than 10 different AAV serotypes and driven by different universal and tissue specific promoters.
Our services include all kind of molecular biology services, such as vector design and construction, gene cloning, gene mutagenesis. Our major services focus on adenovirus, lentivirus and AAV virus production and purification. We have the capacity to prepare more than 80 AAV virus per week, and can produce up to 10E15 AAV viral particles for research use. We also offer cell line engineering, including gene knock out, point mutation, deletion, insertion and fluorescent protein tagging using CRISPR technology.
Working with scientific researchers, we gain the access to unique products. The GRAB sensors for acetylcholine(ACh), dopamine(DA), norepinephrine(NE), Serotonin (5-HT), adenosine(Ado), adenosine triphosphate (ATP), vasoactive intestinal peptide(VIP), cholecystokinin (CCK), endocannabinoid (eCB), neuropeptide Y (NPY), corticotropin-releasing factor (CRF), arginine vasopressin (AVP), histamine (His), somatostatin (SST), melatonin(MT), Anandamide also known as N-arachidonoylethanolamine (AEA), oxytocin (OXT), Histamine(HA) and more. The mouse genome coverage of CRISPR of gRNA clones. DIO and fDIO control gene expression. AAV serotypes selection kit and CRISPR cell based selection systems.
We have many unique and priority technologies, such as 4in1 shRNA, CreOn shRNA and 2in1 gRNA vector constructions. Our optimized cell culture and AAV virus purification protocol enable us to get large amount and high quality of AAV virus in relative short time and less cost. We are also specialized in high through put molecular cloning, have the capacity to generate thousands of cDNA clones, shRNA clones or gRNA clones in a week. Working with customers, we are experiences in rational design and modify AAV CAP gene or build AAV CAP direct evolution library. We have the know-how to run the direct evolution CAP library screen in vitro and in vivo. We have our priority technology in CRISPR gRNA validation in the cell, which can also be used in direct evolution in screen for more powerful CAS9 gene.