Knockout Cell Line
Introduction
In the molecular biology field, the newly developed editing tool, CRISPR/Cas9 system is a powerful tool since 2013, which uses guide RNAs to direct the Cas9 endonuclease to cleave their target sequences. CRISPR Cas9 system is available for precision genome editing: gene knock-out (KO), gene knock-in (KI) and point mutation (PM). KO cell lines generation or KO animal model construction have become very important method for studying gene function and site-directed genome modification. WZ Biosciences offers a full suite of CRISPR Cas9 services, including gRNA design and screening, gRNA/Cas9 vector construction, gRNA/Cas9 viral packaging, KO efficiency validation and KO cell lines generation.
Service Details
WZ Biosciences scientists have years of experience at the generation of KO cell lines, including but not limited to HEK293, HepG2, N2A, A549, PC12, and so on:
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Cat No.
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Type
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KO Cell Line Type
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Price
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Turnaround Time
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WZ120001-1
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Single Gene KO Cell Line Generation
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Polyclonal
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$1500/cell
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4-6weeks
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WZ120001-2
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Monoclonal
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$4500/cell
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12weeks
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WZ120002-1
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Multiple Gene KO cell line Generation
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Polyclonal
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Quote
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Inquire
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WZ120002-2
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Monoclonal
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Quote
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Inquire
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*PS: If you provide KO cell lines to us, additional cost will be required.
* Interested in above KO Cell Line Generation services? Please feel free to contact us to “Request a quote”.
Related services： CRISPR/Cas9 Cloning, Gene Kockout Validation
Service Process
Advantages
Examples
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⊙human AGTR1 KO HEK293 cell
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a: human AGTR1 gene kockdown efficiency determined by PCR
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b：human AGTR1 gene kockdown efficiency determined by Sanger sequencing
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⊙human AKT1 KO HEK293 cell
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a: human AKT1 gene kockdown efficiency determined by PCR
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b：human AKT1 gene kockdown efficiency determined by Sanger sequencing
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Related Resorces
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《Transfection Reagent Instructions》
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