Viral Transduction Enhancer
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一、Product Introduction:
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Product Name: Viral Transduction Enhancer;
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Abbreviation: ADV-HR
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Cat. No:FH880805
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Specification: Concentration 1mg/ mL; Volume 1ml
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Storage conditions: It is recommended to store at -20℃ after aliquoting, and store at 4℃ within a week.
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Reminder: Avoid repeated freezing and thawing! Repeated freezing and thawing will seriously affect the experimental results, please use it within a week!
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二、Product Description：
The Viral Transduction Enhancer(shortened to ADV-HR) enhance virus adsorption on target cell membranes, thereby increasing adenovirus and lentivirus gene transfer efficiency.
三、Viral Transduction Enhancer Protocol ：
3.1 Directly adding the virus to the cell culture, suitable for easy-to-transfect cells
1. For viral transduction, add ADV-HR directly into cell culture dish/well/flask. Shake gently to ensure homogenous spread.
2. The follow-up steps depend on the virus type and individual experiment.
3.2 Incubating cells, virus and ADV-HR before transfering the this mixture to complete medium, suitable for hard-to-transfect cells
Take the 6-well plate as an example:
3.2.1 Preparation of ADV-HR transduction mixtures in a 1.5ml EP tube are given below(Total volume: 100ul):
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① ADV-HR
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X µ L
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② Virus
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Y µ L
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③ Cell suspension
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(100-X-Y) µL （containing 1.0×10E6 cells）
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ADV-HR dosage X refer to pre-experimental results; Virus dosageY is calculated based on virus titer, cell culture formats, and multiplicity of infection (MOI) .
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3.2.2 Mix gently by pipetting up and down, and incubate at 37℃ in for 0.5-1 hours.
3.2.3 Gently resuspend the cells using a pipette, and place directly into the freshly prepared 6-well plate. Gently swirl and place into a 5% CO2 incubator at 37℃.
3.2.4 The follow-up steps depend on the virus type and individual experiment.
四、Examples-The lentiviral transduction efficiency in HepG2 & CNE2 cells with ADV-HR
