Plasmids have become a basic tool in molecular biology due to their simple operation, easy preparation, stable performance and a wide range of applications. Therefore, molecular cloning technology has become an essential laboratory skill for every researcher. Although it is simple, it is fairly laborious. WZ Biosciences is your best choice, with the following features:

The basic molecular cloning usually includes the steps of gene synthesis, restriction digestion, ligation, transformation, clonal selection and verification. The following is an example from ORF AAV plasmid construction to virus packaging. The service flow diagram is as follows:

Step 1：Choose a Right Vector

When choosing a vector, many factors are usually considered by researchers, such as cell type, gene length, and gene characteristic. WZ Biosciences provides the complete gene vectors (viral and non-viral vectors). In addition, you can also send us your own vectors. For some difficult-to-transfect cells, viral vectors are usually the first choice, because they are more efficient than plasmid vectors. Each viral vector system has its merits.

Step 2：Select a Right Promoter

The packaging capacity of viral vectors is a key consideration when choosing a vector. Since they have limited packaging capacity, the success of gene expression is highly dependent on the best suitable promoter choice. The following table lists our tissue-specific promoters. If you do not find the promoter you want to use, you can contact us.

Step 3：Insert an ORF

WZ Biosciences has a collection of over 18,000 Human ORF Clones , which can be shuttled into our destination viral vectors by simple "cut and paste". We can make you desired construct according to your needs. You can choose from our human ORF Clones or just give us the sequence. If you choose our premade clones, please let us know the Cat No., Gene Name and RefSeq No. when you request a quote. In addition, we also provide molecular cloning and site-directed mutagenesis services.

Step 4：Chose a Right Tag

WZ Biosciences offers a complete of gene expression vectors containing different tags, reporters and expression formats. You can customize your desired vectors during AAV cloning, adenovirus cloning, or lentivirus cloning.

Step 5：Package into Viral Particles

If you choose a viral vector and there is a need for viral packaging in the future, then we can directly package the constructed custom vector into viral particles viral particles. Please visit the virus packaging page to learn more about lentivirus, adenovirus and adeno-associated virus packaging.

1. If the customer don't know restriction sites at both ends of the insert sequence and plasmid map, but can provide the sequence and sequencing primer, can we undertake this project?

A: Yes, but we need to sequence to verify the sequence near the restriction site before cloning.

2. Can we provide glycerol bacteria as subcloning template?

A: Not recommended, because glycerol bacteria is not as stable as plasmid, but if glycerol bacteria is the only option, we can also try to do it.

3. The template was previously constructed by WZ Biosciences. Do you still have this plasmid, or do you need us to send it again?

A: We usually keep the constructed plasmid for 1 year. If the previous order has been completed for more than 1 year, but not more than 5 years, we may still have this plasmid, but we need to check with our production team.

4. We have primers, do we need to provide them or do you design and synthesize them yourself?

A: We will design and synthesize primers for cloning by ourselves. You only need to send us cloning template and let us know your desired gene sequence.

5. I have a complicated cloning scheme. Do I still need to design?

A: You just need to provide us template sequence, template plasmid and final sequence, our production team will design the best cloning scheme for you.