Introduction：

WZ Biosciences offers premade 11,000 mouse gRNA pool clones and over 350 human gRNA pool clones. Four gRNAs were designed to use with spCAS9 for each targeting gene. The annealed primers were pooled and cloned into pCAG-GFP-U6-gRNA vector. The gRNA targeting sequences were well selected and tested, we guarantee the knock out efficiency. Our mouse gRNA clones cover most of the functional known genes, such as transcription factors, signal transduction genes, kinase and phosphatase. All our human gRNA pool clones were tested for their knockout function in HEK293 cells, and cover most cancer related gene and markers. Our gRNA clones are compatible with AAV packaging systems. They can be used with spCAS9 transgenic mice for in vivo gene knockout study. All these clones are ready for secondary day delivery.