gRNA library customization service
gRNA libraries are constructed based on the CRISPR/Cas system and can guide Cas nucleases to perform targeted genome editing. The libraries contain a large number of gRNA sequences, enabling targeting of multiple genes or specific pathway genes. Combined with high-throughput screening technologies, they facilitate rapid evaluation of gene functions and interactions, enhancing research efficiency. Lentiviral vectors are commonly used for gRNA library construction due to their ability to efficiently infect various cell types (including hard-to-transfect primary and non-dividing cells) and achieve stable integration of gRNAs into the genome, which supports long-term tracking of gene editing outcomes and large-scale screening experiments.
gRNA library construction process
(Genetic screens in human cells using the CRISPR/Cas9 system)
Weizhen Biosciences provides one-stop gRNA library construction services, fully meeting your library functional screening needs!
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Service Items
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Service Details
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gRNA Library Design and Construction
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gRNA Design, gRNA Oligo Synthesis, Library Plasmid Construction, Library Plasmid Amplification, NGS Quality Control
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Cas9 Stable Cell Line Customization
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Cas9 Lentiviral Packaging, Cas9 Stable Cell Line Construction, Efficacy Validation
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gRNA Library Viral Packaging
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Viral Packaging, Titer Determination
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Functional Screening Experiments
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Cell Viability Screening, Cell Behavior Screening, Drug Screening, etc.
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NGS Analysis
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NGS Sequencing, Bioinformatics Analysis
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Service Advantages
1. High Coverage and Uniform Distribution: Validated by NGS, ensuring library coverage >99% and uniform gRNA distribution to enhance screening efficiency.
2. Flexible Vector Selection: Offers multiple cloning vectors, such as lent-crispr-v2 or other customer-requested vectors, to meet diverse experimental needs.
3. Diverse Delivery Options: Flexible plasmid delivery quantities, ranging from micrograms to milligrams, freely selectable based on experimental scale. Lentivirus and AAV packaging available, with multiple specifications to choose from.
4. Design and Synthesis: Utilizes semiconductor microarray technology for precise single-stranded nucleotide synthesis.
5. Cloning and Validation: Double-stranded gRNAs are cloned into specified vectors, with library coverage and distribution uniformity verified by NGS.
